Peter Nixon
Wolfson Biochemistry Building
Department of Life Sciences
Imperial College London
South Kensington Campus
London. SW7 2AZ, UK
Email: [email protected]
Peter's Publications
2009
Boehm, M., Nield, J., Zhang, P., Aro, E.-M., Komenda, J. and Nixon, P. J. ‘Structural and mutational analysis of the Band 7 proteins of Synechocystis 6803’ J. Bacteriol. (2009) in press
2008
Komenda, J., Nickelsen, J., Tichý, M., Prášil, O., Eichacker, L. A. and Nixon, P. J. ‘The cyanobacterial homologue of HCF136/YCF48 is a component of an early Photosystem II assembly complex and is important for both the efficient assembly and repair of Photosystem II in Synechocystis sp. PCC 6803’ J. Biol. Chem. 283 (2008) 22390-22399.
Schlodder, E., Coleman, W. J., Nixon, P. J., Cohen, R. O., Renger, T. and Diner, B. A. ‘Site-directed mutations at D1-His198 and D1-Thr179 of Photosystem II in Synechocystis sp. PCC 6803: Deciphering the spectral properties of the PSII reaction center’ Phil. Trans. R. Soc. Lond. B 363 (2008) 1197-1202.
Schlodder, E., Renger, T., Raszewski G., Coleman, W. J., Nixon, P. J., Cohen, R. O. and Diner, B. A. ‘Site-Directed Mutations at D1-Thr179 of Photosystem II in Synechocystis sp.PCC 6803 modify the spectroscopic properties of the accessory chlorophyll in the D1-branch of the reaction center’ Biochemistry 47 (2008) 3143-3154.
2007
Cheregi, O., Sicora, C., Kos, P. B., Barker, M., Nixon, P. J. and Vass, I. ‘The role of the FtsH and Deg proteases in the repair of UV-B radiation-damaged Photosystem II in the cyanobacterium Synechocystis PCC 6803’ Biochim. Biophys. Acta Bioenergetics 1767 (2007) 820-828.
Cohen, R. O., Nixon, P. J. and Diner, B. A. ‘Participation of the C-terminal region of the D1 polypeptide in the first steps in the assembly of the Mn4Ca cluster of photosystem II’ J. Biol. Chem. 282 (2007) 7209-7218.
Komenda, J., Kuvikova, S., Granvogl, B., Eichacker, L. A., Diner, B. A. and Nixon, P. J. ‘Cleavage after Ala352 in the C-terminal extension is an early step in the maturation of the photosystem II D1 protein in the cyanobacterium Synechocystis PCC 6803’ Biochim Biophys Acta Bioenergetics 1767 (2007) 829-837.
Komenda, J., Tichý, M., Knoppová, J., Prášil, O., Kuviková, S., de Vries, R. and Nixon, P. J. ‘The exposed N-terminal tail of the D1 subunit is required for rapid D1 degradation during Photosystem II repair’ Plant Cell 19 (2007) 2839-2854.
Zhang, P., Sicora, C. I., Vorontsova, N., Allahverdiyeva, Y., Battchikova, N., Nixon, P. J. and Aro, E.–M. FtsH protease is required for induction of inorganic carbon acquisition complexes in Synechocystis sp. PCC 6803. Mol. Microbiol. 65 (2007) 728-740.
2006
Barker, M., de Vries, R., Nield, J., Komenda, J. and Nixon, P. J. ‘The Deg proteases protect Synechocystis sp. PCC 6803 during heat and light stresses but are not essential for the removal of damaged D1 protein during the photosystem two repair cycle’ J. Biol. Chem. 281 (2006) 30347-30355.
Komenda, J., Barker, M., Kuvikova, S., de Vries, R., Mullineaux, C. W., Tichy, M. and Nixon, P. J. ‘The FtsH protease slr0228 is important for quality control of photosystem two in the thylakoid membrane of Synechocystis PCC 6803’ J. Biol. Chem. 281 (2006) 1145-1151.
2005
Cser, K., Diner, B. A., Nixon, P. J. and Vass, I. ‘The role of D1-Ala344 in charge stabilization and recombination in Photosystem II’ Photochem. Photobiol. Sci. 4 (2005) 1049-1054.
Mussgnug, J. H., Wobbe, L., Elles, I., Claus, C., Hamilton, M., Fink, A., Kahmann, U., Kapazoglou, A., Mullineaux, C. W., Hippler, M., Nickelsen, J., Nixon, P. J. and Kruse, O. ‘NAB1, an RNA-binding protein involved in the light-regulated differential expression of the light-harvesting antenna of Chlamydomonas reinhardtii’ Plant Cell 17 (2005) 3409-3421.
Nixon, P. J., Barker, M., Boehm, M., de Vries, R. and Komenda, J. ‘FtsH-mediated repair of photosystem two in response to light stress’ J. Exp. Bot. 56 (2005) 357-363.
Tregoning, J. S., Clare, S., Bowe, F., Edwards, L., Qazi, O., Nixon, P. J., Maliga, P., Dougan, G. and Hussell, T. ‘Protection against tetanus toxin using a plant-based vaccine’ Eur. J. Immunol. 35 (2005) 1-7.
Yeremenko, N., Jeanjean, R., Prommeenate, P., Krasikov, V., Nixon, P. J., Vermaas, W. F. J., Havaux, M. and Matthijs, H. C. P. ‘ Open reading frame ssr2016 is important for antimycin-sensitive Photosystem I-driven cyclic electron flow in the cyanobacterium Synechocystis sp. PCC 6803’ Plant Cell Physiol. 46 (2005) 1422-1436.
2004
Allahverdiyeva, Y., Deak, Z., Szilard, A., Diner, B. A., Nixon, P. J. and Vass, I. ‘The function of D1-H332 in PSII electron transport studied by thermoluminescence and chlorophyll fluorescence in site-directed mutants of Synechocystis 6803’ Eur. J. Biochem. 271 (2004) 3523-3532.
Diner, B. A., Bautista, J. A., Nixon, P. J. Berthomieu, C., Hinerwadel, R. and Britt, R. D., Vermaas, W. F. J. and Chisholm, D. A. ‘Coordination of proton and electron transfer from the redox-active tyrosine, YZ, of Photosystem II and examination of the electrostatic influence of oxidised tyrosine YD● (H+)’ Phys. Chem. Chem. Phys. 6 (2004) 4844-4850.
Prommeenate, P., Lennon, A. M., Markert, C., Hippler, M. and Nixon, P. J. ‘Subunit composition of NDH-1 complexes of Synechocystis sp. PCC 6803: Identification of two new ndh gene products with nuclear-encoded homologues in the chloroplast Ndh complex’. J. Biol. Chem. 279 (2004) 28165-28173.
Schönfeld, C., Wobbe, L., Borgstädt, R., Kienast, A., Nixon, P. J. and Kruse, O. ‘The nucleus-encoded protein MOC1 is essential for mitochondrial light acclimation in Chlamydomonas reinhardtii’ J. Biol. Chem. 279 (2004) 50366-50374.
Tregoning, J., Maliga, P., Dougan, G. and Nixon, P. J. ‘New advances in the production of edible plant vaccines: chloroplast expression of a tetanus vaccine antigen, TetC’ Phytochemistry 65 (2004) 989-994.
2003
Lennon, A. M., Prommeenate, P. and Nixon, P. J. ‘Location, expression and orientation of the putative chlororespiratory enzymes, Ndh and IMMUTANS, in higher plant plastids’ Planta218 (2003) 254-260.
Silva, P., Thompson, E., Bailey, S., Kruse, O., Mullineaux, C. W., Robinson, C., Mann, N. H. and Nixon, P. J. (2003) ‘FtsH is involved in the early stages of repair of photosystem two in Synechocystis sp. PCC 6803’ Plant Cell 15 (2003) 2152-2164.
Tregoning, J., Nixon, P., Kuroda, H., Svab, Z., Clare, S., Bowe, F., Fairweather, N., Ytterberg, J., van Wijk, K. J., Dougan, G. and Maliga, P. ‘Expression of tetanus toxin Fragment C in tobacco chloroplasts’ Nucl. Acids Res. 31 (2003) 1174-1179.
2002
Bailey, S., Thompson, E., Nixon, P. J., Horton, P., Mullineaux, C. W., Robinson, C and Mann, N. H. ‘A critical role for the Var2 FtsH homologue of Arabidopsis thaliana in the PSII repair cycle in vivo’ J. Biol. Chem. 277 (2002) 2006-2011.
Rappaport, F., Guergova-Kuras, M., Nixon, P. J., Diner, B. A. and Lavergne, J. ‘Kinetics and pathways of charge recombination in Photosystem II’ Biochemistry 41 (2002) 8518-8527
Silva, P., Choi, Y. –J., Hassan, H. A. G. and Nixon, P. J. ‘Involvement of the HtrA family of proteases in the protection of the cyanobacterium Synechocystis PCC 6803 from light stress and in the repair of photosystem two’ Phil. Trans. R. Soc. Lond. B 357 (2002) 1461-1467.
2001
Bailey, S., Silva, P., Nixon, P., Mullineaux, C., Robinson, C. and Mann, N. ‘Auxillary functions in photosynthesis: the role of FtsH’ Biochem Soc Trans 29 (2001) 455-459.
Diner, B. A., Schlodder, E., Nixon, P. J., Coleman, W. J., Rappaport, F., Lavergne, J., Vermaas, W. F. J. and Chisholm, D. A. ‘Site-directed mutations at D1-His198 and D2-His197 of photosystem II in Synechocystis 6803: Sites of primary charge separation and cation and triplet stabilization’ Biochemistry 40 (2001) 9265-9281.
Morais, F., Kühn, K., Stewart, D. H., Barber, J., Brudvig, G. W., and Nixon, P. J. ‘Photosynthetic water oxidation in cytochrome b-559 mutants containing a disrupted haem-binding pocket ’J. Biol. Chem. 276 (2001) 31986-31993.
2000
Campbell, K. A., Force, D. A., Nixon, P. J., Dole, F., Diner, B. A. and Britt, R. D. ‘Dual-mode EPR detects the initial intermediate in photoassembly of the photosystem II Mn cluster: The influence of amino acid residue 170 of the D1 polypeptide on Mn coordination’ J. Am. Chem. Soc. 122 (2000) 3754-3761.
Komenda, J., Hassan, H. A. G., Diner, B. A., Debus, R. J., Barber, J. and Nixon, P.J. ‘Degradation of the Photosystem II D1 and D2 proteins in different strains of the cyanobacterium Synechocystis PCC 6803 varying with respect to the type and level of psbA transcript’ Plant Mol. Biol. 42 (2000) 635-645.
Nixon, P. J. ‘Chlororespiration’ Phil. Trans. R. Soc. Lond. B 355 (2000) 1541-1547.
Stewart, D. H., Nixon, P. J., Diner, B. A. and Brudvig, G. W. ‘Assignment of the Qy absorbance bands of photosystem II chromophores by low temperature optical spectroscopy of wild-type and mutant reaction centers’ Biochemistry 39 (2000) 14583-14594.
1999
Alizadeh, S., Morais, F., Barber, J. and Nixon, P. J. ‘Isotopic labelling of the polypeptide subunits of the isolated photosystem two reaction centre complex of Chlamydomonas reinhardtii suggests an αβ heterodimeric structure for cytochrome b-559’ J. Photobiochem. Photobiol. B: Biol. 48 (1999) 148-153.
Andronis, C., Merry, S. A. P., Durrant, J. R., Klug, D. R., Barber, J. and Nixon, P. J. ‘Mutation of the Chlamydomonas reinhardtii analogue of residue M210 of the Rhodobacter sphaeroides reaction center slows primary electron transfer in photosystem two’ Photosynth. Res. 62 (1999) 205-217.
Kruse, O., Nixon, P. J., Schmid, G. H. and Mullineaux, C. W. ‘Isolation of state transition mutants of Chlamydomonas reinhardtii by fluorescence video imaging’ Photosynth. Res. 61 (1999) 43-51.
1998
Andronis, C., Kruse, O., Deak, Z., Vass, I., Diner, B. A. and Nixon, P. J. ‘Mutation of residue Thr2 of the D2 polypeptide and its effect on photosystem two function in Chlamydomonas reinhardtii’ Plant Physiol. 117 (1998) 515-524.
Burrows, P. A., Sazanov, L. A., Svab, Z., Maliga, P. and Nixon, P. J. ‘Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes’ EMBO J. 17 (1998) 868-876.
Merry, S. A. P., Nixon, P. J., Barter, L. M. C., Schilstra, M., Porter, G., Barber, J., Durrant, J. R. and Klug, D. R. ‘Modulation of quantum yield of primary radical pair formation in photosystem II by site-directed mutagenesis affecting radical cations and anions’ Biochemistry 37 (1998) 17439-17447.
Morais, F., Barber, J. and Nixon, P. J. ‘The chloroplast-encoded alpha subunit of cytochrome b-559 is required for assembly of the photosystem two complex in both the light and the dark’ J. Biol. Chem. 273 (1998) 29315-29320.
Sazanov, L. A., Burrows, P. A. and Nixon, P. J. ‘The plastid ndh genes code for an NADH-specific dehydrogenase: Isolation of a complex I analogue from pea thylakoid membranes’ Proc. Natl. Acad. USA 95 (1998) 1319-1324.
Sazanov, L. A., Burrows, P. A. and Nixon, P. J. ‘The chloroplast Ndh complex mediates the dark reduction of the plastoquinone pool in response to heat stress in tobacco leaves’ FEBS Letts 429 (1998) 115-118.
1997
Dalla Chiesa, M., Friso, G., Deák, Z., Vass, I., Barber, J. and Nixon, P. J. ‘Reduced turnover of the D1 polypeptide and photoactivation of electron transfer in novel herbicide resistant mutants of Synechocystis sp. PCC 6803’ Eur. J. Biochem. 248 (1997) 731-740.
1996
Dalla Chiesa, M., Deák, Z., Vass, I., Barber, J. and Nixon, P. J. ‘The lumenal loop connecting transmembrane helices I and II of the D1 polypeptide is important for assembly of the photosystem two complex’ Photosynth. Res, 50 (1996) 79-91.
Giorgi, L. B., Nixon, P. J., Merry, S. A. P., Joseph, D. M., Durrant, J. R., De Las Rivas, J., Barber, J., Porter, G. and Klug, D. R. ‘Comparison of primary charge separation in the photosystem II reaction centre complex isolated from wild type and D1-130 mutants of the cyanobacterium Synechocystis PCC 6803’ J. Biol. Chem. 271 (1996) 2093-2101.
James, E. K., Iannetta, P. M. M., Nixon, P. J., Whiston, A. J., Peat, L., Crawford, R. M. M., Sprent, J. I. and Brewin, N. J. ‘Photosystem II and oxygen regulation in Sesbania rostrata stem nodules’ Plant Cell and Environment 19 (1996) 895-910.
Sazanov, L. A., Burrows, P. and Nixon, P. J. ‘Detection and characterisation of a complex I-like NADH-specific dehydrogenase from pea thylakoids’ Biochem. Soc. Trans. 24 (1996) 739-743.
1995
Alizadeh, S., Nixon, P. J., Telfer, A. and Barber, J. ‘Isolation and characterisation of the photosystem two reaction centre complex from a double mutant of Chlamydomonas reinhardtii’ Photosynth. Res. 43 (1995) 165-171.
Nixon, P. J. and Diner, B. A. ‘Analysis of water-oxidation mutants constructed in the cyanobacterium Synechocystis sp. PCC 6803’ Biochem. Soc. Trans. 22 (1994) 338-343.
Nixon, P. J., Komenda, J., Barber, J., Deak, Z., Vass, I. and Diner, B. A. ‘Deletion of the PEST-like region of photosystem two modifies the QB-binding pocket but does not prevent rapid turnover of D1’ J. Biol. Chem.270 (1995) 14919-14927.
Vermaas, W., Madsen, C., Yu, J., Visser, J., Metz, J., Nixon, P. J. and Diner, B. A. ‘Turnover of the D1 protein and of photosystem II in a Synechocystis 6803 mutant lacking TyrZ’ Photosynth. Res.45 (1995) 99-104
1994
Alizadeh, S., Nechushtai, R., Barber, J. and Nixon, P. ‘Nucleotide sequence of the psbE, psbF and trnM genes from the chloroplast genome of Chlamydomonas reinhardtii’ Biochim. Biophys. Acta 1188 (1994) 439-442.
Dalla Chiesa, M., Mayes, S. R., Maskell, D. J., Nixon, P. J. and Barber, J. ‘An aroA homologue from Synechocystis sp. PCC 6803’ Gene 144 (1994) 145-146.
Giorgi, L. B., Durrant, J. R., Alizadeh, S., Nixon, P. J., Joseph, D. M., Rech, T., Barber, J., Porter, G. and Klug, D. R. ‘Comparison of primary electron transfer in photosystem two reaction centres isolated from the higher plant Pisum sativum and the green alga Chlamydomonas reinhardtii’ Biochim. Biophys. Acta 1186 (1994) 247-251.
Kruip, J., Nixon, P. J., Osiewacz, H. D. and Rögner, M. ‘Nucleotide sequence of the petB gene encoding cytochrome b6 from the mesophilic cyanobacterium Synechocystis PCC 6803: Implications for evolution and function’ Biochim. Biophys. Acta 1188 (1994) 443-446.
1992
Diner, B. A. and Nixon, P. J. ‘The rate of reduction of oxidized redox-active tyrosine, Z+, by exogenous Mn2+ is slowed in a site-directed mutant, at aspartate 170 of polypeptide D1 of photosystem II, inactive for photosynthetic oxygen evolution’ Biochim. Biophys. Acta1101 (1992) 134-138.
Nixon, P. J. and Diner, B. A. ‘Aspartate-170 of the photosystem II reaction center polypeptide D1 is involved in the assembly of the oxygen-evolving manganese cluster’ Biochemistry 31 (1992) 942-948.
Nixon, P. J., Trost, J. T. and Diner, B. A. ‘Role of the carboxy-terminus of polypeptide D1 in the assembly of a functional water-oxidizing manganese cluster in photosystem II of the cyanobacterium, Synechocystis sp. PCC 6803: Assembly requires a free carboxyl group at C-terminal position 344’ Biochemistry 31 (1992) 10859-10871.
1991
Diner, B. A., Nixon, P. J. and Farchaus, J. W. ‘Site-directed mutagenesis of photosynthetic reaction centers’ Curr. Opin. Struct. Biol. 1 (1991) 546-554.
Nixon, P. J., Rögner, M. and Diner, B. A. ‘Expression of a higher plant psbA gene in Synechocystis 6803 yields a functional hybrid photosystem II reaction center complex’ The Plant Cell 3 (1991) 383-395.
1990
Metz, J., Nixon, P. and Diner, B. ‘Nucleotide sequence of the psbA3 gene from the cyanobacterium Synechocystis PCC 6803’ Nucl. Acids Res. 18 (1990) 6715.
Rögner, M., Nixon, P. J. and Diner, B. A. ‘Purification and Characterisation of Photosystem I and Photosystem II core complexes from wild-type and phycocyanin-deficient strains of the cyanobacterium Synechocystis PCC 6803’ J. Biol. Chem. 265 (1990) 6189-6196.
1989
Metz, J. G., Nixon, P. J., Rögner, M., Brudvig, G. W. and Diner, B. A. ‘Directed alteration of the D1 polypeptide of photosystem II: Evidence that tyrosine-161 is the redox component, Z, connecting the oxygen-evolving complex to the primary electron donor, P680’ Biochemistry 28 (1989) 6960-6969.
Nixon, P. J., Gounaris, K., Coomber, S. A., Hunter, C. N., Dyer T. A. and Barber, J. ‘psbG is not a photosystem two gene but may be an ndh gene’ J. Biol. Chem. 264, (1989) 14129-14135.
1988
Taylor, M. A., Nixon, P. J., Todd, C. M., Barber, J. and Bowyer, J. R. ‘Characterisation of the D1 polypeptide in a Photosystem II mutant (LF-1) of Scenedesmus obliquus blocked on the oxidising side. Evidence supporting non-processing of D1 as the cause of the lesion’ FEBS Letts 235 (1988) 109-116.
1987
Marder, J. B., Chapman, D. J., Telfer, A., Nixon, P. J. and Barber, J. ‘Identification of psbA and psbD gene products, D1 and D2, as reaction center proteins of Photosystem 2’ Plant Mol. Biol. 9 (1987) 325-333.
1986
Nixon, P. J., Dyer, T. A., Barber, J. and Hunter, C. N. ‘Immunological evidence for the presence of the D1 and D2 proteins in PSII cores of higher plants’ FEBS Letts 209 (1986) 83-86.
REVIEWS AND CORRESPONDENCE
Maliga, P. and Nixon, P. J. ‘Judging the homoplastomic state of plastid transformants’ Trends Plant Sci. 3 (1998) 376-377.
Nixon, P. J. and Maliga, P. ‘Chlororespiration: only half a story’ Trends Plant Sci 4 (1999) 51.
CONTRIBUTIONS TO BOOKS EDITED BY OTHERS
Nixon, P. J., Chisholm, D. A. and Diner, B. A. ‘Isolation and functional analysis of random and site-directed mutants of photosystem II’ in ‘Plant Protein Engineering’, Shewry, P. R. and Gutteridge, S. eds., (Cambridge University Press, Cambridge, U.K.) 1992, 93-141.
Nixon, P. and Jansson, C. ‘Cyanobacterial transformation and gene regulation’ in ‘Molecular Genetics of Photosynthesis’, Andersson, B., Salter, A. H. and Barber, J. eds. (Oxford University Press, Oxford, U.K.) 1996, 197-224.
Nixon, P. J. and Mullineaux, C. W. ‘Regulation of photosynthetic electron transport’ in ‘Regulatory Aspects of Photosynthesis’, Andersson, B. and Aro, E.-M. eds. (Kluwer Academic Publishers, Dordrecht, The Netherlands) 2001, pp. 533-555.
Nixon, P. J., Sarcina, M. and Diner, B. A. ‘The D1 and D2 core subunits’ in ‘Photosystem II: The light-driven water:plastoquinone oxidoreductase’, Wydrzynski, T. and Satoh, K. eds. (Kluwer Academic Publishers, Dordrecht, The Netherlands) 2005, pp. 71-94.
Nixon, P. J. and Rich, P. R. ‘Chlororespiratory pathways and their physiological significance’ in ‘The Structure and Function of Plastids’, Wise R. R. and Hoober, K. eds. (Kluwer Academic Publishers, Dordrecht, The Netherlands) 2006, pp. 237-251.
Battchikova, N., Aro, E.-M. and Nixon, P. J. ‘Structure and physiological function of NDH-1 complexes in cyanobacteria’ in ‘The Bioenergetic processes of cyanobacteria’, Peschek, G. ed., (Kluwer Academic Publishers, Dordrecht, The Netherlands), in press
PATENTS
‘High level Expression of Immunogenic Proteins in the Plastids of Higher Plants’. Inventors: Pal Maliga, Gordon Dougan, Peter Nixon and John Tregoning. PCT/US03/09970 (WO 03/083086 A2) U.S. Patent Application Serial No. 10/109,812.