Prof. Michael Hippler

Institute of Plant Biochemistry and Biotechnology
The University of Munster, Germany
Phone: +49 251 83 24790
Fax: +49 251 83 28371
Email: [email protected]

Michael Hippler

Michael's Publications


Clemens, S., Naumann, B. and Hippler, M. (2009) Proteomics of metal mediated protein dynamics in plants - iron and cadmium in the focus. Frontiers in Biosciences, 14, 1955-1969.

Stauber, E.J., Busch, A., Naumann, B., Svatos, A. and Hippler, M. (2009) Proteotypic profiling of LHCI from Chlamydomonas reinhardtii provides new insights into structure and function of the complex. Proteomics, 9, 398-408.

Rolland, N., Atteia, A., Decottignies, P., Garin, J., Hippler, M., Kreimer, G., Lemaire, S.D., Mittag, M. and Wagner, V. (2009) Chlamydomonas proteomics. Curr Opin Microbiol, 12, 285-291.

Ozawa, S.I., Nield, J., Terao, A., Stauber, E.J., Hippler, M., Koike, H., Rochaix, J.D. and Takahashi, Y. (2009) Biochemical and Structural Studies of the Large Ycf4-Photosystem I Assembly Complex of the Green Alga Chlamydomonas reinhardtii. Plant Cell., in press

Petroutsos, D., Terauchi, A.M., Busch, A., Hirschmann, I., Merchant, S.S., Finazzi, G. and Hippler, M. (2009) PGRL1 participates in iron-induced remodeling of the photosynthetic apparatus and in energy metabolism in Chlamydomonas reinhardtii. J Biol Chem, in press.


Weinl, S., Held, K., Schlucking, K., Steinhorst, L., Kuhlgert, S., Hippler, M. and Kudla, J. (2008) A plastid protein crucial for Ca2+-regulated stomatal responses. New Phytol, 179, 675-686.

Busch, A., Rimbauld, B., Naumann, B., Rensch, S. and Hippler, M. (2008) Ferritin is required for rapid remodeling of the photosynthetic apparatus and minimises photo-oxidative stress in response to iron availability in Chlamydomonas reinhardtii. Plant J, 55, 201-211.

Allmer, J., Kuhlgert, S. and Hippler, M. (2008) 2DB: a Proteomics database for storage, analysis, presentation, and retrieval of information from mass spectrometric experiments. BMC Bioinformatics, 9, 302.


Sarry, J.E., Chen, S., Collum, R.P., Liang, S., Peng, M., Lang, A., Naumann, B., Dzierszinski, F., Yuan, C.X., Hippler, M. and Rea, P.A. (2007) Analysis of the vacuolar luminal proteome of Saccharomyces cerevisiae. Febs J. 274: 4287-4305.

Merchant, S.S., Prochnik, S.E., Vallon, O., Harris, E.H., Karpowicz, S.J., et al. (2007) The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science, 318, 245-250.

Naumann, B., Busch, A., Allmer, J., Ostendorf, E., Zeller, M., Kirchhoff, H. and Hippler, M. (2007) Comparative quantitative proteomics to investigate the remodeling of bioenergetic pathways under iron deficiency in Chlamydomonas reinhardtii. Proteomics, 7, 3964-3979.


Onder, O., Yoon, H., Naumann, B., Hippler, M., Dancis, A. and Daldal, F. (2006) Modifications of the Lipoamide-containing Mitochondrial Subproteome in a Yeast Mutant Defective in Cysteine Desulfurase. Mol Cell Proteomics 5: 1426-1436.

Sommer, F., Drepper, F., Haehnel, W. and Hippler, M. (2006) Identification of Precise Electrostatic Recognition Sites between Cytochrome c6 and the Photosystem I Subunit PsaF Using Mass Spectrometry. J Biol Chem 281: 35097-35103.

Allmer, J., Naumann, B., Markert, C., Zhang, M. and Hippler, M. (2006) Mass spectrometric genomic data mining: Novel insights into bioenergetic pathways in Chlamydomonas reinhardtii. Proteomics 6: 6207-6220.


Naumann, B., Stauber, E.J., Busch, A., Sommer, F. and Hippler, M. (2005) N-terminal processing of Lhca3 Is a key step in remodeling of the photosystem I-light-harvesting complex under iron deficiency in Chlamydomonas reinhardtii. J Biol Chem 280: 20431-20441.

Finazzi, G., Sommer, F. and Hippler, M. (2005) Release of oxidized plastocyanin from photosystem I limits electron transfer between photosystem I and cytochrome b6f complex in vivo. Proc Natl Acad Sci USA 102: 7031-7036.

Mussgnug, J.H., Wobbe, L., Elles, I., Claus, C., Hamilton, M., Fink, A., Kahmann, U., Kapazoglou, A., Mullineaux, C.W., Hippler, M., Nickelsen, J., Nixon, P.J. and Kruse, O. (2005) NAB1 Is an RNA Binding Protein Involved in the Light-Regulated Differential Expression of the Light-Harvesting Antenna of Chlamydomonas reinhardtii. Plant Cell 17: 3409-3421.


Wagner, V., Fiedler, M., Markert, C., Hippler, M. and Mittag, M. (2004) Functional proteomics of circadian expressed proteins from Chlamydomonas reinhardtii. FEBS Lett 559: 129-135.

Allmer, J., Markert, C., Stauber, E.J. and Hippler, M. (2004) A new approach that allows identification of intron-split peptides from mass spectrometric data in genomic databases. FEBS Lett 562: 202-206.

Sommer, F., Drepper, F., Haehnel, W. and Hippler, M. (2004) The hydrophobic recognition site formed by residues PsaA-W651 and PsaB-W627 of photosystem I in Chlamydomonas reinhardtii confers distinct selectivity for binding of plastocyanin and cytochrome c6. J Biol Chem. 279: 12009-12017.

Wittstock, U., Agerbirk, N., Stauber, E.J., Olsen, C.E., Hippler, M., Mitchell-Olds, T., Gershenzon, J. and Vogel, H. (2004) Successful herbivore attack due to metabolic diversion of a plant chemical defense. Proc Natl Acad Sci USA 101: 4859-4864.

Reimann, R., Hippler, M., Machelett, B. and Appenroth, K.J. (2004) Light Induces Phosphorylation of Glucan Water Dikinase, Which Precedes Starch Degradation in Turions of the Duckweed Spirodela polyrhiza. Plant Physiol 135: 121-128.

Takahashi, Y., Yasui, T.A., Stauber, E.J. and Hippler, M. (2004) Comparison of the Subunit Compositions of the PSI-LHCI Supercomplex and the LHCI in the Green Alga Chlamydomonas reinhardtii. Biochemistry 43: 7816-7823.

Storf, S., Stauber, E.J., Hippler, M. and Schmid, V.H. (2004) Proteomic analysis of the photosystem I light-harvesting antenna in tomato (Lycopersicon esculentum). Biochemistry 43: 9214-9224.

Nield, J., Redding, K. and Hippler, M. (2004) Remodeling of light-harvesting protein complexes in chlamydomonas in response to environmental changes. Eukaryot Cell 3: 1370-1380.

Stauber, E.J. and Hippler, M. (2004) Chlamydomonas reinhardtii proteomics. Plant Physiol Biochem 42: 989-1001.

Sayre, R.T. and Hippler, M. (2004) Introduction: Molecular genomics of the Chlamydomonas chloroplast. Photosynth Res 82: 201-202.


Stauber, E.J., Fink, A., Markert, C., Kruse, O., Johanningmeier, U. and Hippler, M. (2003) Proteomics of Chlamydomonas reinhardtii Light-Harvesting Proteins. Eukaryot Cell 2: 978-994.

Sommer, F., Hippler, M., Biehler, K., Fischer, N. and Rochaix, J.D. (2003) Comparative analysis of photosensitivity in photosystem I donor and acceptor side mutants of Chlamydomonas reinhardtii. Plant Cell and Environment 26: 1881-1892.


Sommer, F., Drepper, F. and Hippler, M. (2002) The luminal Helix l of PsaB is essential for recognition of plastocyanin or cytochrome c6 and fast electron transfer to photosystem I in Chlamydomonas reinhardtii. J. Biol. Chem. 277: 6573-81.

Moseley, J.L., Page, M.D., Pergam, N., Eriksson, M., Quinn, J., Soto, J., Theg, S., Hippler, M. and Merchant, S. (2002) Reciprocal expression of two di-iron enzymes affecting photosystem I and light-harvesting complex accumulation. Plant Cell 14: 673-688.

Hippler, M., Rimbault, B. and Takahashi, Y. (2002) Photosynthetic complex assembly in Chlamydomonas reinhardtii. Protist 153: 197-220.

Moseley, J.L., Allinger, T., Herzog, S., Hoerth, P., Wehinger, E., Merchant, S. and Hippler, M. (2002) Adaptation to Fe-deficiency requires re-modelling of the phoosynthetic apparatus. EMBO J. 21: 6709-6720.


Hippler, M., Klein, J., Fink, T., Allinger, T. and Hoerth, P. (2001) Towards functional proteomics of membrane protein complexes: Analysis of thylakoid membranes from Chlamydomonas reinhardtii. Plant J. 28: 595-606.


Hippler, M., Biehler, K., Krieger-Liszkay, A., van Dillewjin, J. and Rochaix, J.D. (2000) Limitation in electron transfer in photosystem I donor side mutants of Chlamydomonas reinhardtii. Lethal photo-oxidative damage in high light is overcome in a suppressor strain deficient in the assembly of the light harvesting complex. J. Biol. Chem. 275: 5852-5859.

Rochaix, J., Fischer, N. and Hippler, M. (2000) Chloroplast site-directed mutagenesis of photosystem I in Chlamydomonas: electron transfer reactions and light sensitivity. Biochimie 82: 635-645.


Hippler, M., Drepper, F, Rochaix, J.D. and Muehlenhoff, U. (1999) Insertion of the N-terminal Part of PsaF from Chlamydomonas reinhardtii into photosystem I from Synechococcus elongatus enables efficient binding of algal plastocyanin and cytochrome c6. J. Biol. Chem. 274: 4180-4188.

Fischer, N., Boudreau, E., Hippler, M., Drepper, F., Haehnel, W. and Rochaix, J.D. (1999) A large fraction of PsaF is nonfunctional in photosystem I complexes lacking the PsaJ subunit. Biochemistry 38: 5546-5552.


Fischer N., Hippler, M., Setif, P., Jacquot, J.-P. and Rochaix, J.D. (1998) The PsaC subunit of Photosystem I provides an essential lysine residue for fast electron transfer to ferredoxin. EMBO J. 16: 849-858.

Hippler, M., Drepper, F., Haehnel, W. and Rochaix, J.D. (1998) The N-terminal domain of PsaF: Precise recognition site for binding and fast electron transfer from cytochrome c6 and plastocyanin to photosystem I of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA 95: 7339-7344.

Hippler, M., Redding, K. and Rochaix, J.D. (1998) Chlamydomonas genetics: A tool for the study of bioenergetic pathways. Biochim. Biophys. Acta 1367: 1-62.

1997 Hippler, M., Drepper, F., Farah, J. and Rochaix, J.D. (1997) Fast electron transfer from cytochrome c6 and plastocyanin to photosystem I of Chlamydomonas reinhardtii requires PsaF. Biochemistry 36: 6343-6349.


Hippler, M., Riedel, A., Schroer, U., Nitschke, W. and Haehnel, W. (1996) Light-induced charge separation between plastocyanin and the iron-sulfur clusters FA and FB in the complex of plastocyanin and photosystem I. Arch Biochem Biophys 330: 414-418.

Drepper, F., Hippler, M., Nitschke, W. and Haehnel, W. (1996) Binding dynamics and electron transfer between plastocyanin and photosystem I. Biochemistry 35: 1282-1295.

Hippler, M., Reichert, J., Sutter M., Zak, E., Altschmied, L., Schröer, U., Herrmann, R.G. and Haehnel, W. (1996) The plastocyanin binding domain of photosystem I. EMBO J. 15: 6374-6384.


Hippler, M., Ratajczak, R. and Haehnel, W. (1989) Identification of the plastocyanin binding subunit of photosystem I. FEBS Lett. 250: 280-284.

Book Articles

B. Naumann and M. Hippler (2007) Insights into chloroplast proteomics: from basic principles to new horizons. In Topics in Current Genetics Ed. R.Bock, Springer-Verlag, in press

F Drepper and M Hippler (2006) Binding and electron transfer between photosystem I and plastocyanin or cytochrome c6. In Advances in Photosynthesis and Respiration Springer-Verlag, in press

M Hippler and R Bock (2004) Extranuclear inheritance: Chloroplast Proteomics. In Progress in Botany. Springer-Verlag Berlin Heidelberg, Vol. 65, pp. 90-105

R Bock and M Hippler (2002) Extranuclear inheritance: Functional genomics in chloroplasts. In: Progress in Botany, 63, Springer-Verlag, Berlin Heidelberg, pages 106-131

F Sommer and M Hippler (2002) Photosystem I: Structure/Function and Assembly of a transmembrane light-driven Plastocyanin/Cytochrome c6 - Ferredoxin Oxidoreductase. Handbook of Photochemistry and Photobiology Vol. 4, Ed. H.S. Nalwa, American Scientific Publishers, Stevenson Ranch, California, USA, pages 269-294